Plant Tissue Culture

[prajjwal]

Mean while you people can check this videos published by Commercial labs to perform PTC

Media preparation:
http://www.youtube.com/watch?v=ltbdM3boWmU

Aseptic technique:
http://www.youtube.com/watch?v=yJ_acpKg ... re=related

Asectic culture transfer from solid media to liquid media:
http://www.youtube.com/watch?v=krZl4KKzYF8

And with Plants:
http://www.youtube.com/watch?v=zd0iVJrQwyY

Let me know if you have any quarries related to these videos.

Cheers.

[maaz]

nothing available here....guys update anyone have something related to these..

[prajjwal]

nothing available here......

What availability?


Now get some idea about the media components needs for PTC. Note: Different plants need different media composition. Their are different types of Media for individual plants. For example, Rooting Media, Shooting media, Callus media, Liquid single cell media and Complete media.

Check this video for some details about the ingredients you need to make a PTC Media (also known as MS Media)

http://www.youtube.com/watch?v=zBLW0XG4 ... re=related


Got fear?? Commercial base media is also available. I idea about the price, but it can be arranged if you people want. Info: 1 Commercial PTC Unit is located in IMT Manesar. But not allowed visit.

[prajjwal]

Here we can get some ready made media for PTC

http://www.sigmaaldrich.com/life-scienc ... lines.html

May be at a premium price. Let me find the other sources

[prajjwal]

Now, we should move into the main discussion. Those above posts ware to give an Idea about the process and short of introduction of the topic.



Following things we will need to set up a Plant tissue culture facility in our home.

1. An area where we can do Aseptic culture transfer. Preferably a 3x2x2 qft 5 side closed area and with a smooth surface for easy cleaning option. Their must be a good lighting source at the top and an UV lamp for surface and air sterilization. The front should be closed upto 75% with an Acrylic sheet and rest of the open area can be cover with hinge fitted acrylic sheet.

2. Spirit lamp or Bunsen Burner with LPG fittings.

3. Cuttings tools, like forcep, scalpel, needle and scissors

4. Ethanol (70%) for surface cleaning

5. Mercuric chloride solution, 5% Bleaching solution for plant body sterilization

6. Commercial growth Media (MS) and Hormone (Auxin)

7. 5 litter pressure cooker for Sterilization

8. Non absorbent cotton for plugging, containers to keep the plants (Autoclave safe). Preferably polypropylene make.

9. Racks to keep those container after inoculation. Lighting fittings on top with T5 (6500k), Osram fluora

10. Temp 20-25*C in grow-up room


NB: Remind me if I have missed anything

[maaz]

nothing available here......

What availability?

this kind of lab setup..nothing...

[maaz]

wow gud info and very nice sharing i like to do it now any one else like to come with me

[maaz]

Here we can get some ready made media for PTC

http://www.sigmaaldrich.com/life-scienc ... lines.html

May be at a premium price. Let me find the other sources

In this which media is good for the aquatic plants..

[maaz]

Here is my little research regarding media Rajiv Ji what media you used in past..

Macronutrients
Ammonium nitrate (NH4NO3) 1,650 mg/l
Calcium chloride (CaCl2 · 2H2O) 440 mg/l
Magnesium sulfate (MgSO4 · 7H2O) 370 mg/l
Potassium phosphate (KH2PO4) 170 mg/l
Potassium nitrate (KNO3) 1,900 mg/l



Micronutrients
Boric acid (H3BO3) 6.2 mg/l
Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l
Cupric sulfate (CuSO4 · 5H2O) 0.025 mg/l
Ferrous sulfate (FeSO4 · 7H2O) 27.8 mg/l
Manganese sulfate (MnSO4 · 4H2O) 22.3 mg/l
Potassium iodide (KI) 0.83 mg/l
Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l
Zinc sulfate (ZnSO4·7H2O) 8.6 mg/l
Na2EDTA · 2H2O 37.2 mg/l


Common organic additives
i-Inositol 100 mg/l
Niacin 0.5 mg/l
Pyridoxine · HCl 0.5 mg/l
Thiamine · HCl 0.1 mg/l
IAA 1–30 mg/l
Kinetin 0.04–10 mg/l
Glycine (recrystallized) 2.0 mg/l
Edamine S 1.0 g/l
Sucrose 20 g/l
Agar 10 g/l

[prajjwal]

Here we can get some ready made media for PTC

http://www.sigmaaldrich.com/life-scienc ... lines.html

May be at a premium price. Let me find the other sources

In this which media is good for the aquatic plants..

Little problem in that... I do not have expertness in PTC. Just did some Lab work during my Post Graduation and that is also 8 years back! Hardly remember the media composition. As I mentioned earlier, University note books could help me a lot to even designing a suitable media for Aquatic plants. Considering our aquatic plant a soft tissue one, we can try with any Complete media to start with. According to the response from the plant, we can modify the components we need. Adding Auxin hormone will do the rest.

Above link is only for reference, not for putting an order. Other cheaper option will get available shortly.


Regards,

Requesting members to participate in this discussion to Guide me.

Addition with the list mentioned earlier:
11. Weighing scale (0.1mg precision)
12. Measuring cylinder
13. Laboratory grade distilled water
14. Surgical gloves


............................................