easy way out for ph value.

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deepesh
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Re: easy way out for ph value.

Unread post by deepesh »

Hi prajjwal

This is just for knowledge as I am not using a pH meter. Do the calibrating buffer solutions degrade at room temps? Is that why you recommend the refrigerator?

I use pH strips which perform fine for freshwater fish and are really quite superfluous in my setting.
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saikumar
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Re: easy way out for ph value.

Unread post by saikumar »

deepesh wrote:Hi prajjwal

This is just for knowledge as I am not using a pH meter. Do the calibrating buffer solutions degrade at room temps? Is that why you recommend the refrigerator?

I use pH strips which perform fine for freshwater fish and are really quite superfluous in my setting.
same question, on the stock solution I got, its not mentioned. Moreover it says 4 readings at 4 diff temperature caliberations.
prajjwal
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Re: easy way out for ph value.

Unread post by prajjwal »

Most of the buffer solutions are mainly combination of one part week acid (eg. citric acid, phosphoric acid etc) and one part of salt of that acid. Sometimes one part of week acid salt and one part of di-hydrate salt of same base in different combination. Though its not necessary to keep all buffer solution in refrigerated condition, but most of them should kept in 4*C of even some in -20*C to prevent formation of more complex compound which then become cause error. Specially when you don't know whats inside the bottle.

Normally commercially available buffer solutions doesn't comes with a level "keep refrigerated" but one should.

Hard to tell shelf life, but when I was working in R&D of a commercial lab, we use to discard stock buffer solution every month even refrigerated condition storing. Otherwise calibration error use to occur.

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deepesh
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Re: easy way out for ph value.

Unread post by deepesh »

@saikumar
The four different values are what prajjwal was alluding to. Temperature changes pH of buffer solutions.

@prajjwal
Thanks for the info. I had no idea at all.
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prajjwal
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Re: easy way out for ph value.

Unread post by prajjwal »

Most welcome... I tried to simplify as much as I could.

One more point (Not sure that I have mentioned or not - during calibration of pH meter, bring the temperature of Buffer solution same with the target sample for more accuracy.

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Re: easy way out for ph value.

Unread post by Joforing »

one more question, during calibration each time should we wash the ph meter probe with ph 7(distilled water) solution??
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Re: easy way out for ph value.

Unread post by prajjwal »

Yes... with distilled water and wipe with good quality tissue paper after any dip in any solution, so that the distilled water drop would not alter the pH of buffer solution. Also it avoids cross interference with another buffer.

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Re: easy way out for ph value.

Unread post by Joforing »

thank u so much for ur guidance...
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Re: easy way out for ph value.

Unread post by prajjwal »

Welcome :)

Please let me know if you need any more info... will try to answer

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